Details

Autor(en) / Beteiligte

• Ha, Suk-Jin
• Kim, Sang-Yong
• Seo, Jin-Ho
• Oh, Deok-Kun
• Lee, Jung-Kul

Titel

Optimization of culture conditions and scale-up to pilot and plant scales for coenzyme Q₁₀ production by Agrobacterium tumefaciens

Ist Teil von

• Applied microbiology and biotechnology, 2007-04, Vol.74 (5), p.974-980

Ort / Verlag

Berlin: Berlin/Heidelberg : Springer-Verlag

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• This report describes the optimization of culture conditions for coenzyme Q₁₀ (CoQ₁₀) production by Agrobacterium tumefaciens KCCM 10413, an identified high-CoQ₁₀-producing strain (Kim et al., Korean patent. 10-0458818, 2002b). Among the conditions tested, the pH and the dissolved oxygen (DO) levels were the key factors affecting CoQ₁₀ production. When the pH and DO levels were controlled at 7.0 and 0-10%, respectively, a dry cell weight (DCW) of 48.4 g l-¹ and a CoQ₁₀ production of 320 mg l-¹ were obtained after 96 h of batch culture, corresponding to a specific CoQ₁₀ content of 6.61 mg g-DCW-¹. In a fed-batch culture of sucrose, the DCW, specific CoQ₁₀ content, and CoQ₁₀ production increased to 53.6 g l-¹, 8.54 mg g-DCW-¹, and 458 mg l-¹, respectively. CoQ₁₀ production was scaled up from a laboratory scale (5-l fermentor) to a pilot scale (300 l) and a plant scale (5,000 l) using the impeller tip velocity (V tip) as a scale-up parameter. CoQ₁₀ production at the laboratory scale was similar to those at the pilot and plant scales. This is the first report of pilot- and plant-scale productions of CoQ₁₀ in A. tumefaciens.