Details

Autor(en) / Beteiligte

• Franklin, Peter
• Moeller, Alexander
• Hall, Graham L.
• Horak, Friedrich
• Patterson, Hilary
• Stick, Stephen M.

Titel

Variability of nitric oxide metabolites in exhaled breath condensate

Ist Teil von

• Respiratory medicine, 2006, Vol.100 (1), p.123-129

Ort / Verlag

Oxford: Elsevier Ltd

Erscheinungsjahr

2006

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• The collection of exhaled breath condensate (EBC) is simple and non-invasive, however, there are few data on the methodological aspects affecting concentrations of compounds in EBC. The aim of this study was to investigate methodological issues for measuring nitric oxide metabolites (NO x ) in EBC. Twenty-five healthy adults (12 females, age range 23–55 years) and 22 children (11 females, age range 7–6 years) were recruited for studies investigating inter- and intra-day repeatability, repeatability with controlled expiratory flows and temperature, flow dependence, and analytical variability of EBC NO x . Both intra- and inter-day repeatability was poor with a coefficient of repeatability of 103.4% of the mean difference between intra-day (15 min) measures and 118.6% of inter-day (24 h) differences. Repeatability was not improved when expiratory flow and temperature of the collection device were controlled. However, some of the variability (approximately 50%) may be accounted for by variability in the analytical technique (analytical variability) and this may result from difficulties in controlling for contamination. NO x levels were not affected by different expiratory flows in either adults or children but there was still significant variation within individuals. Levels of NO x in EBC seem to be highly variable and this needs to be considered if EBC NO x is to be used in clinical studies.