Details

Autor(en) / Beteiligte

• Wang, Yu-Lai
• Liu, Wanzhao
• Sun, Ying-Jie
• Kwon, Jungkee
• Setsuie, Rieko
• Osaka, Hitoshi
• Noda, Mami
• Aoki, Shunsuke
• Yoshikawa, Yasuhiro
• Wada, Keiji

Titel

Overexpression of ubiquitin carboxyl-terminal hydrolase L1 arrests spermatogenesis in transgenic mice

Ist Teil von

• Molecular reproduction and development, 2006-01, Vol.73 (1), p.40-49

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

2006

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Ubiquitin carboxyl‐terminal hydrolase 1 (UCH‐L1) can be detected in mouse testicular germ cells, mainly spermatogonia and somatic Sertoli cells, but its physiological role is unknown. We show that transgenic (Tg) mice overexpressing EF1α promoter‐driven UCH‐L1 in the testis are sterile due to a block during spermatogenesis at an early stage (pachytene) of meiosis. Interestingly, almost all spermatogonia and Sertoli cells expressing excess UCH‐L1, but little PCNA (proliferating cell nuclear antigen), showed no morphological signs of apoptosis or TUNEL‐positive staining. Rather, germ cell apoptosis was mainly detected in primary spermatocytes having weak or negative UCH‐L1 expression but strong PCNA expression. These data suggest that overexpression of UCH‐L1 affects spermatogenesis during meiosis and, in particular, induces apoptosis in primary spermatocytes. In addition to results of caspases‐3 upregulation and Bcl‐2 downregulation, excess UCH‐L1 influenced the distribution of PCNA, suggesting a specific role for UCH‐L1 in the processes of mitotic proliferation and differentiation of spermatogonial stem cells during spermatogenesis. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.