Details

Autor(en) / Beteiligte

• Deng, Xinxiu
• Chen, Xiaohui
• Yin, Ran
• Shen, Zhenduo
• Qiao, Lei
• Bi, Kaishun

Titel

Determination of deoxyschizandrin in rat plasma by LC–MS

Ist Teil von

• Journal of pharmaceutical and biomedical analysis, 2008-01, Vol.46 (1), p.121-126

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• A simple, rapid and sensitive LC–MS method was developed for quantification of deoxyschizandrin in rat plasma. A 50 μl plasma sample was extracted by ether and performed on Elite Hypersil C 18 column (200 mm × 4.6 mm, 5 μm) with the mobile phase of methanol–water (84:16, v/v) in a run time of 6.5 min. The analyte was monitored with positive atmospheric pressure chemical ionization (APCI) by selected ion monitoring (SIM) mode. A good linear relationship was obtained over the range of 1.0–50.0 ng/ml and the validated method was successfully applied for the pharmacokinetic studies of deoxyschizandrin in rat. After oral administration of 4 mg/kg deoxyschizandrin and Schisandra extract which contained the same dose of deoxyschizandrin to male rats, the C max of deoxyschizandrin were 15.8 ± 3.1 and 34.3 ± 16.8 ng/ml, T max were 0.51 ± 0.13 and 3.83 ± 1.83 h, T 1/2 were 5.3 ± 2.2 and 6.5 ± 3.4 h.