Molecular immunology, 2008-02, Vol.45 (4), p.1027-1034
2008
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Details
- Autor(en) / Beteiligte
- Titel
- Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2F5
- Ist Teil von
- Molecular immunology, 2008-02, Vol.45 (4), p.1027-1034
- Ort / Verlag
- England: Elsevier Ltd
- Erscheinungsjahr
- 2008
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Anti-idiotypic antibody (Ab2) 3H6 is directed against the human monoclonal antibody 2F5, which is one of a few neutralising antibodies against HIV-1. Since the binding epitope of 2F5 is cryptic and no neutralising immune response could be elicited by several potential vaccines comprising this region, Ab2/3H6 represents a potent vaccine candidate for active immunisation. Here we describe the molecular features of Ab2/3H6 after changing the antigen binding specificity by single point mutations in the complementarity-determining region 3 of the Ab2/3H6 heavy chain. The resulting Ab2/3H6 mutants were compared in several experimental settings to the wild type Ab2/3H6 Fab fragment. Moreover, we report about an immunisation study with Ab2/3H6 Fab variants, which elicited a specific 2F5-like humoral immune response in BALB/c mice.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0161-5890eISSN: 1872-9142DOI: 10.1016/j.molimm.2007.07.030Titel-ID: cdi_proquest_miscellaneous_70088335
- Format
- –
- Schlagworte
- 2F5, Animals, Anti-idiotypic antibodies, Antibodies, Anti-Idiotypic - administration & dosage, Antibodies, Anti-Idiotypic - genetics, Antibodies, Anti-Idiotypic - immunology, Antibodies, Monoclonal - immunology, Antibody Formation, CHO Cells, Complementarity Determining Regions - administration & dosage, Complementarity Determining Regions - genetics, Complementarity Determining Regions - immunology, Cricetinae, Cricetulus, ELDKWA, Epitopes, Female, HIV-1, Human immunodeficiency virus 1, Humans, Immunoglobulin Fab Fragments - administration & dosage, Immunoglobulin Fab Fragments - genetics, Immunoglobulin Fab Fragments - immunology, Immunoglobulin Heavy Chains - administration & dosage, Immunoglobulin Heavy Chains - genetics, Immunoglobulin Heavy Chains - immunology, Mice, Mice, Inbred BALB C, Point Mutation, Vaccine