Details

Autor(en) / Beteiligte

• Marchese, Annalisa
• Bošković, Radovan I
• Caruso, Tiziano
• Raimondo, Antonio
• Cutuli, Marcello
• Tobutt, Kenneth R

Titel

new self-compatibility haplotype in the sweet cherry 'Kronio', S₅', attributable to a pollen-part mutation in the SFB gene

Ist Teil von

• Journal of experimental botany, 2007, Vol.58 (15-16), p.4347-4356

Ort / Verlag

Oxford: Oxford University Press

Erscheinungsjahr

2007

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• 'Kronio' is a Sicilian cultivar of sweet cherry (Prunus avium), nominally with the incompatibility genotype S₅S₆, that is reported to be naturally self-compatible. In this work the cause of its self-compatibility was investigated. Test selfing confirmed self-compatibility and provided embryos for analysis; PCR with consensus primers designed to amplify S-RNase and SFB alleles showed that the embryos were of two types, S₅S₅ and S₅S₆, indicating that S₆ pollen failed, but S₅ succeeded, perhaps because of a mutation in the pollen or stylar component. Stylar RNase analysis indicated active S-RNases for both S₅ and S₆. The S-RNase alleles were cloned and sequenced; and sequences encode functional proteins. Cloning and sequencing of SFB alleles showed that S₆ was normal but S₅ had a premature stop codon upstream of the variable region HVa resulting in a truncated protein. Therefore, the self-compatibility can be attributed to a pollen-part mutation of S₅, designated S₅', the first reported case of breakdown of self-incompatibility in diploid sweet cherry caused by a natural mutation at the S-locus. The second intron of the S-RNase associated with S₅' contained a microsatellite smaller than that associated with S₅; primers designed to amplify across this microsatellite effectively distinguished S₅ from S₅'. Analysis of some other Sicilian cherries with these primers indicated that S₅' is also present in the Sicilian cultivar 'Maiolina a Rappu', and this proved to be self-compatible.