Details

Autor(en) / Beteiligte

• Kaser, Arthur
• Dunzendorfer, Stefan
• Offner, Felix A
• Ryan, Thomas
• Schwabegger, Anton
• Cruikshank, William W
• Wiedermann, Christian J
• Tilg, Herbert

Titel

A Role for IL-16 in the Cross-Talk Between Dendritic Cells and T Cells

Ist Teil von

• The Journal of immunology (1950), 1999-09, Vol.163 (6), p.3232-3238

Ort / Verlag

United States: Am Assoc Immnol

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• Dendritic cells (DCs) in the periphery capture and process Ags, migrate to lymphoid organs, and initiate immune responses in T cells. IL-16, the soluble ligand for CD4, is a potent chemoattractant for CD4+ T cells, eosinophils, and monocytes and is mainly derived from activated T cells. Because migration is a fundamental property of DCs, we asked whether IL-16 induces chemotaxis in DCs and whether DCs are a source of IL-16. DCs were generated by culture of monocytes in IL-4 and GM-CSF for 6 days and subsequently highly purified employing magnetic beads. Migration was assayed by nitrocellulose and polycarbonate filter-based assays, and distinction of chemotaxis and chemokinesis was performed by a checkerboard analysis. Messenger RNA and protein data revealed constitutive expression and release of IL-16 by day-6 DCs. Gradients of rIL-16 induced a chemotactic response of DCs. Furthermore, the chemotactic activity of DC supernatant toward DCs themselves and T cells was mainly due to IL-16, because the addition of neutralizing Abs completely abrogated the migratory response. However, after induction of maturation by the addition of TNF-alpha and PGE2 DCs, neither expressed IL-16 mRNA nor produced IL-16 protein. We conclude that IL-16 may play a role in the trafficking of DCs and may be a major chemotactic signal from DCs toward themselves and toward T cells.