Details

Autor(en) / Beteiligte

• Purdie, Neil
• Province, Dennis W
• Layloff, Thomas P
• Nasr, Moheb M

Titel

Algorithms for Validating Chiral Properties of Insulins

Ist Teil von

• Analytical chemistry (Washington), 1999-08, Vol.71 (16), p.3341-3346

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• The combination of chiral ligand exchange on Cu(II) complexes in aqueous base with circular dichroism spectropolarimetric detection provides excellent avenues to validate the chirality properties of oligopeptides and proteins. The method is quick and simple and has the potential for development into an automated, routine procedure for quality control applications. Target analytes used for this first study of a protein system are human, porcine, and bovine insulins prepared by different procedures and obtained from different sources, production lots, and manufacturers. The analytical specificity of the test makes the method a potentially useful technique for validating the chirality properties of many peptide and protein forms.