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Algorithms for Validating Chiral Properties of Insulins
Ist Teil von
Analytical chemistry (Washington), 1999-08, Vol.71 (16), p.3341-3346
Ort / Verlag
Washington, DC: American Chemical Society
Erscheinungsjahr
1999
Quelle
MEDLINE
Beschreibungen/Notizen
The combination of chiral ligand exchange on Cu(II) complexes in aqueous base with circular dichroism spectropolarimetric detection provides excellent avenues to validate the chirality properties of oligopeptides and proteins. The method is quick and simple and has the potential for development into an automated, routine procedure for quality control applications. Target analytes used for this first study of a protein system are human, porcine, and bovine insulins prepared by different procedures and obtained from different sources, production lots, and manufacturers. The analytical specificity of the test makes the method a potentially useful technique for validating the chirality properties of many peptide and protein forms.