Antiviral research, 1999-07, Vol.42 (3), p.177-187
1999
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- Autor(en) / Beteiligte
- Titel
- A new contained human immunodeficiency virus type 1 host cell system for evaluation of antiviral activities of interferons and other agents in vitro
- Ist Teil von
- Antiviral research, 1999-07, Vol.42 (3), p.177-187
- Ort / Verlag
- Amsterdam: Elsevier B.V
- Erscheinungsjahr
- 1999
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- HIV-host infection systems in vitro are important in the pre-clinical assessment of anti-retroviral drug activity. The present report describes the development of a new HIV-host model comprised of an epithelial cell line of HeLa lineage (HeLa-1), transfected with expression vectors bearing tat and rev (TART) genes of HIV-1 as well as the CD4 receptor gene, and HIV-1 ΔTat/Rev, a biologically contained strain of HIV-1 deleted in tat and rev. Measurement of infectivity, by syncytium formation and reverse transcriptase assay, revealed that HeLa-1 is infected with HIV-1 ΔTat/Rev. This virus failed to productively infect the TART-deficient CD4-positive HeLa cells, confirming its contained, non-infectious nature. The HeLa-1/HIV-1 ΔTat/Rev system was used to measure the anti-retroviral activity of a human leukocyte-derived interferon (IFN-αn3) preparation, several nucleoside analogs, and protease inhibitors. The HeLa-1/HIV-1 ΔTat/Rev model provides a biologically contained system for the study of the HIV pathogenesis and the relative and combined therapeutic effects of anti-retroviral agents in vitro.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0166-3542eISSN: 1872-9096DOI: 10.1016/S0166-3542(99)00014-5Titel-ID: cdi_proquest_miscellaneous_69949587
- Format
- –
- Schlagworte
- AIDS/HIV, Anti-HIV Agents - pharmacology, Antibiotics. Antiinfectious agents. Antiparasitic agents, Antiviral, Antiviral agents, Biological and medical sciences, CD4 Antigens - genetics, Drug Evaluation, Preclinical - methods, Epithelial cells, Gene Expression, Gene Products, rev - genetics, Gene Products, tat - genetics, HeLa Cells, HIV, HIV Protease Inhibitors - pharmacology, HIV-1 - drug effects, Human immunodeficiency virus 1, Humans, IFN, In Vitro Techniques, Interferon-alpha - pharmacology, Medical sciences, Nelfinavir - pharmacology, Pharmacology. Drug treatments, rev Gene Products, Human Immunodeficiency Virus, Reverse Transcriptase Inhibitors - pharmacology, Ritonavir - pharmacology, Saquinavir - pharmacology, tat Gene Products, Human Immunodeficiency Virus, Zidovudine - pharmacology