Details

Autor(en) / Beteiligte

• Tomsik, Pavel
• Sispera, Ludek
• Rezacova, Martina
• Niang, Mohamed
• Stoklasova, Alena
• Cerman, Jaroslav
• Knizek, Jiri
• Brcakova, Eva
• Cermanova, Jolana
• Micuda, Stanislav

Titel

Increased melibiose/rhamnose ratio in bile of rats with acute cholestasis

Ist Teil von

• Journal of gastroenterology and hepatology, 2008-12, Vol.23 (12), p.1934-1940

Ort / Verlag

Melbourne, Australia: Blackwell Publishing Asia

Erscheinungsjahr

2008

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Background and Aim:  Melibiose/rhamnose permeability test is used for noninvasive intestinal mucosa barrier testing. However, the possible escape route of the absorbed saccharides through either intact or impaired blood–biliary barriers has not so far been explored. The objective of the present study was therefore two‐fold: First, to describe in detail the biliary pharmacokinetics of melibiose and rhamnose in rats; second, to evaluate the changes of both sugars' pharmacokinetics upon impairment of the blood–biliary barrier by acute extrahepatic cholestasis in rats. Methods:  Bile duct obstructed (BDO), sham‐operated and intact (unoperated) male Wistar rats were administered, 24 h after the appropriate intervention, with a single intravenous dose of melibiose and rhamnose, and a 4‐h pharmacokinetic study was performed. Results:  In intact animals, the biliary excretion of melibiose and rhamnose was only 0.06% and 0.4% of the administered dose, respectively, while the urinary excretion accounted for 70.6% and 61.7%, respectively. In BDO animals, the biliary excretion rate of both saccharides, especially that of melibiose, was increased with a consequent 4.4‐fold rise of the biliary melibiose/rhamnose ratio, the accepted paracellular permeability indicator. Both, the renal clearance of melibiose and the urinary melibiose/rhamnose ratio remained uninfluenced by cholestasis. Conclusion:  The present study is the first to describe in detail pharmacokinetic parameters and the biliary excretion of melibiose and rhamnose in healthy and cholestatic rats. The altered melibiose/rhamnose biliary excretion ratio in BDO rats indicates that the test is able to detect the impairment of the blood–biliary barrier in acute extrahepatic cholestasis.