Zhong hua yi xue za zhi, 2008-08, Vol.88 (31), p.2204-2208
2008
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- Autor(en) / Beteiligte
- Titel
- Adenovirus mediated angiostatin gene therapy for ovarian cancer: experiment with nude mice
- Ist Teil von
- Zhong hua yi xue za zhi, 2008-08, Vol.88 (31), p.2204-2208
- Ort / Verlag
- China
- Erscheinungsjahr
- 2008
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To built an expression vector of angiostatin (AG) gene with recombinated replication defective adenovirus and investigate the therapeutic effect of human AG gene on ovarian cancer. (1) Human AG K (1 - 3) cDNA was inserted into the vector pShuttle to build the recombinant plasmid pShttle-AG (K1-3). pAdeno-X-AG (K1-3) was built by double-cut and recombinated pShttle-AG (K1-3) to vector pAdeno-X, and then recombinant adenovirus was finally prepared by transfection of pAdeno-X-AG (K1-3) into to the human embryo kidney cells of the line 293. (2) Human ovarian cancer cells of the line SKOV3 were inoculated subcutaneously into nude mice of the line BALB/c nu/nu to establish model of human ovarian cancer. Then the mice were randomly divided into 3 groups to be injected with Ad = AG (K1-3), Ad-LacZ, or phosphate buffered saline (PBS) around the cancer every 5 days. The tumor size was measured every 5 days to calculate the tumor volume and tumor inhibition rate. Three days after the last injection the mice were killed.
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0376-2491Titel-ID: cdi_proquest_miscellaneous_69899909
- Format
- –
- Schlagworte
- Adenoviridae - genetics, Angiostatins - genetics, Angiostatins - physiology, Animals, Cell Line, Cell Line, Tumor, Female, Genetic Therapy - methods, Genetic Vectors, Humans, Mice, Mice, Inbred BALB C, Mice, Nude, Ovarian Neoplasms - pathology, Ovarian Neoplasms - therapy, Random Allocation, Tumor Burden, Xenograft Model Antitumor Assays