Details

Autor(en) / Beteiligte

• Shimo, Tsuyoshi
• Nakanishi, Tohru
• Nishida, Takashi
• Asano, Masahiro
• Kanyama, Manabu
• Kuboki, Takuo
• Tamatani, Takuya
• Tezuka, Katsunari
• Takemura, Motohide
• Matsumura, Tomohiro
• Takigawa, Masaharu

Titel

Connective Tissue Growth Factor Induces the Proliferation, Migration, and Tube Formation of Vascular Endothelial Cells In Vitro, and Angiogenesis In Vivo

Ist Teil von

• Journal of biochemistry (Tokyo), 1999-07, Vol.126 (1), p.137-145

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• Connective tissue growth factor (CTGF) is a novel cysteine-rich, secreted protein. Recently, we found that inhibition of the endogenous expression of CTGF by its antisense oligonu-cleotide and antisense RNA suppresses the proliferation and migration of vascular endo-thelial cells. In the present study, the following observations demonstrated the angiogenic function of CTGF in vitro and in vivo: (i) purified recombinant CTGF (rCTGF) promoted the adhesion, proliferation and migration of vascular endothelial cells in a dose-dependent manner under serum-free conditions, and these effects were inhibited by anti-CTGF antibodies; (ii) rCTGF markedly induced the tube formation of vascular endothelial cells, and this effect was stronger than that of basic fibroblast growth factor or vascular endothelial growth factor; (iii) application of rCTGF to the chicken chorioallantoic membrane resulted in a gross angiogenic response, and this effect was also inhibited by anti-CTGF antibodies. (iv) rCTGF injected with collagen gel into the backs of mice induced strong angiogenesis in vivo. These findings indicate that CTGF is a novel, potent angiogenesis factor which functions in multi-stages in this process.