Details

Autor(en) / Beteiligte

• BUNNELL, B. A
• KLUGE, K. A
• DONAHUE, R. E
• LEE-LIN, S.-Q
• BYRNE, E. R
• ORLIC, D
• METZGER, M. E
• AGRICOLA, B. A
• WERSTO, R. P
• BODINE, D. M
• MORGAN, R. A

Titel

Transplantation of transduced nonhuman primate CD34+ cells using a gibbon ape leukemia virus vector : restricted expression of the gibbon ape leukemia virus receptor to a subset of CD34+ cells

Ist Teil von

• Gene therapy, 1999, Vol.6 (1), p.48-56

Ort / Verlag

Basingstoke: Nature Publishing Group

Erscheinungsjahr

1999

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The transduction efficiencies of immunoselected rhesus macaque (Macaca mulatta) CD34+ cells and colony-forming progenitor cells based on polymerase chain reaction (PCR) analysis were comparable for an amphotropic Moloney murine leukemia virus (MLV) retroviral vector and a retroviral vector derived from the gibbon ape leukemia virus (GaLV) packaging cell line, PG13. On performing autologous transplantation studies using immunoselected CD34+ cells transduced with the GaLV envelope (env) retroviral vector, less than 1% of peripheral blood (PB) contained provirus. This was true whether bone marrow (BM) or cytokine-mobilized PB immunoselected CD34+ cells were reinfused. This level of marking was evident in two animals whose platelet counts never fell below 50,000/microliter and whose leukocyte counts had recovered by days 8 and 10 after having received 1.7 x 10(7) or greater of cytokine-mobilized CD34+ PB cells/kg. Reverse transcriptase(RT)-PCR analysis of CD34+ subsets for both the GaLV and amphotropic receptor were performed. The expression of the GaLV receptor was determined to be restricted to CD34+ Thy-1+ cells, and both CD34+ CD38+ and CD34+ CD38dim cells, while the amphotropic receptor was present on all CD34+ cell subsets examined. Our findings suggest that, in rhesus macaques, PG13-derived retroviral vectors may only be able to transduce a subset of CD34+ cells as only CD34+ Thy-1+ cells express the GaLV receptor.