Details

Autor(en) / Beteiligte

• Xu, Rong
• Deng, Kejing
• Zhu, Yi
• Wu, Yue
• Ren, Jing
• Wan, Min
• Zhao, Shouyuan
• Wu, Xiaohui
• Han, Min
• Zhuang, Yuan
• Xu, Tian

Titel

A large-scale functional approach to uncover human genes and pathways in Drosophila

Ist Teil von

• Cell research, 2008-11, Vol.18 (11), p.1114-1127

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• We demonstrate the feasibility of performing a systematic screen for human gene functions in Drosophila by assaying for their ability to induce overexpression phenotypes. Over 1 500 transgenic fly lines corresponding to 236 human genes have been established. In all, 51 lines are capable of eliciting a phenotype suggesting that the human genes are functional. These heterologous genes are functionally relevant as we have found a similar mutant phenotype caused either by a dominant negative mutant form of the human ribosomal protein L8 gene or by RNAi downregulation of the Drosophila RPL8. Significantly, the Drosophila RPL8 mutant can be rescued by wild-type human RPL8. We also provide genetic evidence that Drosophila RPL8 is a new member of the insulin signaling pathway. In summary, the functions of many human genes appear to be highly conserved, and the ability to identify them in Drosophila represents a powerful genetic tool for large-scale analysis of human transcripts in vivo.