Details

Autor(en) / Beteiligte

• Gribkoff, Valentin K.
• Pieschl, Rick L.
• Wisialowski, Todd A.
• Park, Won K.
• Strecker, George J.
• de Jeu, Marcel T. G.
• Pennartz, Cyriel M. A.
• Dudek, F. Edward

Titel

A Reexamination of the Role of GABA in the Mammalian Suprachiasmatic Nucleus

Ist Teil von

• Journal of biological rhythms, 1999-04, Vol.14 (2), p.126-130

Ort / Verlag

Thousand Oaks, CA: Sage Publications

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• Three independent electrophysiological approaches in hypothalamic slices were used to test the hypothesis that [.gamma]-amino butyric acid (GABA)Areceptor activation excites suprachiasmatic nucleus (SCN) neurons during the subjective day, consistent with a recent report. First, multiple-unit recordings during either the subjective day or night showed that GABA or muscimol inhibited firing activity of the SCN population in a dose-dependent manner. Second, cell-attached recordings during the subjective day demonstrated an inhibitory effect of bath-or microapplied GABA on action currents of single SCN neurons. Third, gramicidin perforated-patch recordings showed that bicuculline increased the spontaneous firing rate during the subjective day. Therefore, electrophysiological data obtained by three different experimental methods provide evidence that GABA is inhibitory rather than excitatory during the subjective day.