Details

Autor(en) / Beteiligte

• Vikholm-Lundin, Inger
• Pulli, Timo
• Albers, Willem M.
• Tappura, Kirsi

Titel

A comparative evaluation of molecular recognition by monolayers composed of synthetic receptors or oriented antibodies

Ist Teil von

• Biosensors & bioelectronics, 2008-12, Vol.24 (4), p.1036-1038

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• Recombinant anti-morphine Fab′ fragments have been immobilised on gold by covalent attachment through the free thiol groups of the fragment. The antibody fragments were intercalated with a non-ionic hydrophilic polymer in order to suppress non-specific binding of interfering substances. The antibodies are oriented on the surface due to the thiol groups of the antibody and the layer shows a high response to antigen. Non-specific binding of bovine serum albumin is moreover very low because of the repellent polymer. Synthetic receptors composed of an imprinted self-assembled monolayer made from lipoates and the template, morphine, exhibit the same binding response to the antigen, morphine as the site-specific oriented antibody monolayer. A similar binding curve could be obtained as that for binding of morphine to an antibody Fab′ fragment/polymer layer – indicating that synthetic receptors produced are comparable to those of antibody layers. Concentrations down to 0.1 ng/ml have been measured with surface plasmon resonance.