FEBS letters, 1999-03, Vol.446 (1), p.55-59
1999
Details
- Autor(en) / Beteiligte
- Titel
- Hypoxia increases the association of 4E-binding protein 1 with the initiation factor 4E in isolated rat hepatocytes
- Ist Teil von
- FEBS letters, 1999-03, Vol.446 (1), p.55-59
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 1999
- Link zum Volltext
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Incubation of hepatocytes under hypoxia increases binding of translation initiation factor eIF-4E to its inhibitory regulator 4E-BP1, and this correlates with dephosphorylation of 4E-BP1. Rapamycin induced the same effect in aerobic cells but no additive effect was observed when hypoxic cells were treated with rapamycin. This enhanced association of 4E-BP1 with eIF-4E might be mediated by mTOR. Nevertheless, only hypoxia produces a rapid inhibition of protein synthesis. Although hypoxia might be signalling via the rapamycin-sensitive pathway by changing eIF-4E availability, such a pathway is unlikely to be responsible for the depression in overall protein synthesis under hypoxia.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/S0014-5793(99)00185-4Titel-ID: cdi_proquest_miscellaneous_69657367
- Format
- –
- Schlagworte
- 4E-BP, eIF-4E-binding protein, Animals, Antifungal Agents - pharmacology, BSA, bovine serum albumin, Carrier Proteins, Cell Hypoxia, Cells, Cultured, eIF, eukaryotic initiation factor, Eukaryotic initiation factor, Eukaryotic Initiation Factor-4E, Hypoxia, Liver - metabolism, Liver - pathology, m7GTP, 7-methylguanosine triphosphate, Male, mTOR, mammalian target of rapamycin, p70S6K, 70-kDa S6 protein kinase, PAGE, polyacrylamide gel electrophoresis, Peptide Initiation Factors - metabolism, PHAS-I, phosphoprotein heat- and acid-stable induced by insulin, Phosphoprotein heat- and acid-stable induced by insulin, Phosphoproteins - metabolism, Phosphorylation, PMSF, phenylmethylsulfonyl fluoride, Protein synthesis, Rats, Rats, Wistar, Signal Transduction, Sirolimus - pharmacology