Details

Autor(en) / Beteiligte

• van der Klei, Ida J
• Badger, Jonathan H
• Albang, Richard
• Nijland, Jeroen G
• Wagner, Christian
• van den Berg, Marco A
• M Driessen, Arnold J
• Roubos, Johannes A
• van Peij, Noël N M E
• Albermann, Kaj
• von Döhren, Hans
• Garcia-Estrada, Carlos
• Joardar, Vinita
• Wortman, Jennifer
• Martín, Juan F
• W Kiel, Jan A K
• Daran, Jean-Marc
• Veenhuis, Marten
• Bovenberg, Roel A L
• Kovalchuk, Andriy
• Pronk, Jack T
• Harris, Diana M
• Nierman, William C
• Heijne, Wilbert H M
• Fedorova, Natalie D

Titel

Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum

Ist Teil von

• Nature biotechnology, 2008-10, Vol.26 (10), p.1161-1168

Ort / Verlag

New York: Nature Publishing Group US

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• Penicillins and derived β-lactam antibiotics are essential in healthcare. To gain more insight into penicillin synthesis van den Berg and colleagues sequence and analyze the genome and transcriptome of the filamentous fungus Penicillium chrysogenum . Industrial penicillin production with the filamentous fungus Penicillium chrysogenum is based on an unprecedented effort in microbial strain improvement. To gain more insight into penicillin synthesis, we sequenced the 32.19 Mb genome of P. chrysogenum Wisconsin54-1255 and identified numerous genes responsible for key steps in penicillin production. DNA microarrays were used to compare the transcriptomes of the sequenced strain and a penicillinG high-producing strain, grown in the presence and absence of the side-chain precursor phenylacetic acid. Transcription of genes involved in biosynthesis of valine, cysteine and α-aminoadipic acid—precursors for penicillin biosynthesis—as well as of genes encoding microbody proteins, was increased in the high-producing strain. Some gene products were shown to be directly controlling β-lactam output. Many key cellular transport processes involving penicillins and intermediates remain to be characterized at the molecular level. Genes predicted to encode transporters were strongly overrepresented among the genes transcriptionally upregulated under conditions that stimulate penicillinG production, illustrating potential for future genomics-driven metabolic engineering.