Details

Autor(en) / Beteiligte

• Jasiak, Anna J.
• Hartmann, Holger
• Karakasili, Elena
• Kalocsay, Marian
• Flatley, Andrew
• Kremmer, Elisabeth
• Strässer, Katja
• Martin, Dietmar E.
• Söding, Johannes
• Cramer, Patrick

Titel

Genome-associated RNA Polymerase II Includes the Dissociable Rpb4/7 Subcomplex

Ist Teil von

• The Journal of biological chemistry, 2008-09, Vol.283 (39), p.26423-26427

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2008

Quelle

MEDLINE

Beschreibungen/Notizen

• Yeast RNA polymerase (Pol) II consists of a 10-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immunoprecipitation coupled to high resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle.