Details

Autor(en) / Beteiligte

• Laan, Martti
• Cui, Zhi-Hua
• Hoshino, Hiroshi
• Lotvall, Jan
• Sjostrand, Margareta
• Gruenert, Dieter C
• Skoogh, Bengt-Eric
• Linden, Anders

Titel

Neutrophil Recruitment by Human IL-17 Via C-X-C Chemokine Release in the Airways

Ist Teil von

• The Journal of immunology (1950), 1999-02, Vol.162 (4), p.2347-2352

Ort / Verlag

United States: Am Assoc Immnol

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• IL-17 is a recently discovered cytokine that can be released from activated human CD4+ T lymphocytes. This study assessed the proinflammatory effects of human (h) IL-17 in the airways. In vitro, hIL-17 increased the release of IL-8 in human bronchial epithelial and venous endothelial cells, in a time- and concentration-dependent fashion. This effect of hIL-17 was inhibited by cotreatment with an anti-hIL-17 Ab and was potentiated by hTNF-alpha. In addition, hIL-17 increased the expression of hIL-8 mRNA in bronchial epithelial cells. Conditioned medium from hIL-17-treated bronchial epithelial cells increased human neutrophil migration in vitro. This effect was blocked by an anti-hIL-8 Ab. In vivo, intratracheal instillation of hIL-17 selectively recruited neutrophils into rat airways. This recruitment of neutrophils into the airways was inhibited by an anti-hIL-17 Ab and accompanied by increased levels of rat macrophage inflammatory protein-2 (rMIP-2) in bronchoalveolar lavage (BAL) fluid. The BAL neutrophilia was also blocked by an anti-rMIP-2 Ab. The effect of hIL-17 on the release of hIL-8 and rMIP-2 was also inhibited by glucocorticoids, in vitro and in vivo, respectively. These data demonstrate that hIL-17 can specifically and selectively recruit neutrophils into the airways via the release of C-X-C chemokines from bronchial epithelial cells and suggest a novel mechanism linking the activation of T-lymphocytes to recruitment of neutrophils into the airways.