Details

Autor(en) / Beteiligte

• Loft, Steffen
• Poulsen, Henrik Enghusen

Titel

Markers of oxidative damage to DNA: Antioxidants and molecular damage

Ist Teil von

• Methods in Enzymology, 1999, Vol.300, p.166-184

Ort / Verlag

United States: Elsevier Science & Technology

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• More than 100 different oxidative modifications have been observed in DNA. However, so far only a few of the base modifications have been used as biomarkers, and of these, the oxidative C-8 adduct of guanine is by far the most studied as either the nucleoside or base. In principle, the level in DNA from target or surrogate tissues or cells or the excretion of repair products into the urine can be measured. Under the usual steady-state conditions, the latter will reflect the rate of damage, whereas the former will reflect the balance between damage and repair. The chapter discusses the involved assays, the interpretation of the methods, and some results from antioxidant intervention studies, particularly in humans. The assays for the urinary DNA repair products include high-performance liquid chromatography (HPLC) with electrochemical detection (EC) for 8-oxodG and 8-oxoGua and with ultra violet (UV) absorbance detection for thymidine glycol (dTg) and thymine glycol (Tg), whereas all the repair products can potentially be measured by gas chromatography-mass spectrometry (GC/MS). The major problem with all these assays involves separation of the very small amounts of analyte from urine, which is a very complicated matrix.