Details

Autor(en) / Beteiligte

• Kinoshita, Hiroyuki
• Iwahashi, Shizue
• Kakutani, Tetsuya
• Mizumoto, Kazuhiro
• Iranami, Hiroshi
• Hatano, Yoshio

Titel

The Role of Endothelium-Derived Nitric Oxide in Relaxations to Levcromakalim in the Rat Aorta

Ist Teil von

• Japanese Journal of Pharmacology, 1999, Vol.81 (4), p.362-366

Ort / Verlag

Japan: The Japanese Pharmacological Society

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• The present study was designed to examine the role of basally released nitric oxide in relaxations to an ATP-sensitive K+ channel opener. Whether relaxations to levcromakalim are modulated by endothelial removal or the inhibitors of vasodilator effects of endothelium-derived nitric oxide, were investigated in the rat aorta. During contractions to phenylephrine (3 × 10−7 to 10−6 M), levcromakalim (10−8 to 10−5 M) or a nitric oxide donor, l-hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3-methyl-l-triazene (NOC-7,10−9 to 10−5 M), was added in a cumulative fashion. Relaxations to levcromakalim (10−8 to 10−5 M) were significantly reduced by the endothelium-removal. In aortas with endothelium, relaxations in response to levcromakalim were decreased by selective inhibitors of nitric oxide synthase (NG-nitro-L-arginine methyl ester, 10−4M) and soluble guanylate cyclase (lH-[l,2,4]oxadiazolo[4,3-a]quinoxaline-l-one; ODQ, 10−5 M) and a scavenger of nitric oxide (carboxy-PTIO, 10−3 M). Relaxations to levcromakalim in aortas treated with these inhibitors are comparable to those seen in aortas without endothelium. KC1 (30 mM) and an ATP-sensitive K+ channel inhibitor, glibenclamide (10−5 M), abolished relaxations to levcromakalim in aortas with or without endothelium, whereas glibenclamide did not alter relaxations to NOC-7 (10−9 to 10−5 M) in aortas without endothelium. These results suggest that in rat aortas, inhibition of vasodilator effects of basally released nitric oxide can reduce relaxations via ATP-sensitive K+ channels, although these channels do not mediate relaxations to exogenously applied nitric oxide.