Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Recognition of a human sperm surface protein involved in the progesterone-initiated acrosome reaction by antisera against an endomembrane progesterone binding protein from porcine liver
Ist Teil von
Molecular and cellular endocrinology, 1999-12, Vol.158 (1), p.187-193
Ort / Verlag
Ireland: Elsevier Ireland Ltd
Erscheinungsjahr
1999
Quelle
Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
Beschreibungen/Notizen
Antisera against a porcine liver endomembrane progesterone (P
4)-binding protein inhibited the P
4-initiated acrosome reaction (AR) but not the ionomycin-initiated AR of human sperm. Indirect immunofluorescence studies detected antigen in the sperm head that moved during capacitation from a posterior head region to a midhead region. Moreover, the antisera detected a 44.6 kDa protein in western blots of sperm digitonin extracts. These results suggest that a sperm protein with at least partial homology to the liver endomembrane P
4-binding protein, is a putative P
4-receptor on the sperm plasma membrane involved in the P
4-initiated AR.