Molecular and cellular endocrinology, 1999-12, Vol.158 (1), p.187-193
1999
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Details
- Autor(en) / Beteiligte
- Titel
- Recognition of a human sperm surface protein involved in the progesterone-initiated acrosome reaction by antisera against an endomembrane progesterone binding protein from porcine liver
- Ist Teil von
- Molecular and cellular endocrinology, 1999-12, Vol.158 (1), p.187-193
- Ort / Verlag
- Ireland: Elsevier Ireland Ltd
- Erscheinungsjahr
- 1999
- Quelle
- Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
- Beschreibungen/Notizen
- Antisera against a porcine liver endomembrane progesterone (P 4)-binding protein inhibited the P 4-initiated acrosome reaction (AR) but not the ionomycin-initiated AR of human sperm. Indirect immunofluorescence studies detected antigen in the sperm head that moved during capacitation from a posterior head region to a midhead region. Moreover, the antisera detected a 44.6 kDa protein in western blots of sperm digitonin extracts. These results suggest that a sperm protein with at least partial homology to the liver endomembrane P 4-binding protein, is a putative P 4-receptor on the sperm plasma membrane involved in the P 4-initiated AR.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0303-7207eISSN: 1872-8057DOI: 10.1016/S0303-7207(99)00173-2Titel-ID: cdi_proquest_miscellaneous_69393729
- Format
- –
- Schlagworte
- Acrosome reaction, Acrosome Reaction - physiology, Animals, Antisera, Blotting, Western, Fluorescent Antibody Technique, Humans, Immune Sera, Liver (P 4)-binding protein, Liver - metabolism, Male, Membrane Proteins - immunology, Membrane Proteins - metabolism, Progesterone, Progesterone - metabolism, Progesterone-Binding Globulin - immunology, Progesterone-Binding Globulin - metabolism, Sperm, Sperm Capacitation - physiology, Sperm Head - metabolism, Spermatozoa - metabolism, Swine