Details

Autor(en) / Beteiligte

• Hara, Hirokazu
• Ohta, Mitsuhiro
• Ohta, Kiyoe
• Nishimura, Masataka
• Obayashi, Hiroshi
• Adachi, Tetsuo

Titel

Isolation of Two Novel Alternative Splicing Variants of Allograft Inflammatory Factor-1

Ist Teil von

• Biological chemistry, 1999-11, Vol.380 (11), p.1333-1336

Ort / Verlag

Germany: Walter de Gruyter

Erscheinungsjahr

1999

Quelle

MEDLINE

Beschreibungen/Notizen

• Allograft inflammatory factor-1 (AIF-1) was initially cloned from rat cardiac allografts undergoing chronic rejection. AIF-1, possessing an EF-hand-like motif, is thought to be a Ca2+ binding protein. In this study, we identified two novel alternatively spliced variants of AIF-1 by reverse-transcription polymerase chain reaction. One variant encodes an AIF-1 protein that lacks 14 amino acids corresponding to one exon. The other variant encodes a truncated AIF-1 protein due to a frameshift introduced by an 85-bp insertion, and its C-terminal region differs from that of AIF-1. Interestingly, these variants have incomplete and no EF-hand-like motifs, respectively. The expression level of the latter variant is high in peripheral blood leukocytes, and it is selectively expressed in macrophage-like cell lines.