Nature genetics, 2008-08, Vol.40 (8), p.971-976
2008
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- Autor(en) / Beteiligte
- Titel
- Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians
- Ist Teil von
- Nature genetics, 2008-08, Vol.40 (8), p.971-976
- Ort / Verlag
- New York: Nature Publishing Group US
- Erscheinungsjahr
- 2008
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Wolf Reik and Ian Dunham and colleagues cloned and sequenced the complete IGF2-H19 locus in tammar wallaby, a marsupial. Functional analyses revealed conservation of imprinting mechanisms, including germline DNA methylation, between marsupials and eutherians. Comparisons between eutherians and marsupials suggest limited conservation of the molecular mechanisms that control genomic imprinting in mammals. We have studied the evolution of the imprinted IGF2-H19 locus in therians. Although marsupial orthologs of protein-coding exons were easily identified, the use of evolutionarily conserved regions and low-stringency Bl2seq comparisons was required to delineate a candidate H19 noncoding RNA sequence. The therian H19 orthologs show miR-675 and exon structure conservation, suggesting functional selection on both features. Transcription start site sequences and poly(A) signals are also conserved. As in eutherians, marsupial H19 is maternally expressed and paternal methylation upstream of the gene originates in the male germline, encompasses a CTCF insulator, and spreads somatically into the H19 gene. The conservation in all therians of the mechanism controlling imprinting of the IGF2-H19 locus suggests a sequential model of imprinting evolution.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1061-4036eISSN: 1546-1718DOI: 10.1038/ng.168Titel-ID: cdi_proquest_miscellaneous_69370606
- Format
- –
- Schlagworte
- Agriculture, Animal Genetics and Genomics, Animals, Base Sequence, Biological and medical sciences, Biomedical and Life Sciences, Biomedicine, Breastfeeding & lactation, Cancer Research, Conservation, Councils, DNA Methylation, Evolution, Molecular, Female, Fundamental and applied biological sciences. Psychology, Gene Function, Genetics of eukaryotes. Biological and molecular evolution, Genomic Imprinting, Human Genetics, Humans, Insulin-Like Growth Factor II - genetics, Insulin-like growth factors, Macropodidae - genetics, Male, Medical research, Mice, Molecular Sequence Data, Nucleic Acid Conformation, Physiological aspects, Ribonucleic acid, Ribosomal RNA, RNA, RNA, Long Noncoding, RNA, Untranslated - chemistry, RNA, Untranslated - genetics, Sequence Alignment, Structure, Studies, Tissues