Details

Autor(en) / Beteiligte

• Cook, James C.
• Joyce, Joseph G.
• George, Hugh A.
• Schultz, Loren D.
• Hurni, William M.
• Jansen, Kathrin U.
• Hepler, Robert W.
• Ip, Charlotte
• Lowe, Robert S.
• Keller, Paul M.
• Lehman, E.Dale

Titel

Purification of Virus-like Particles of Recombinant Human Papillomavirus Type 11 Major Capsid Protein L1 from Saccharomyces cerevisiae

Ist Teil von

• Protein expression and purification, 1999-12, Vol.17 (3), p.477-484

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1999

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Recombinant major capsid protein, L1 (Mr = 55,000), of human papillomavirus type 11 was expressed intracellularly at high levels in a galactose-inducible Saccharomyces cerevisiae expression system by an HPV6/11 hybrid gene. The capsid protein self-assembled into virus-like particles (VLPs) and accounted for 15% of the total soluble protein. A purification process was developed that consisted of two main steps: microfiltration and cation-exchange chromatography. The purified VLPs were 98% homogeneous, and the overall purification yield was 10%. The final product was characterized by several analytical methods and was highly immunogenic in mice.