Details

Autor(en) / Beteiligte

• Branderhorst, Hilbert M
• Ruijtenbeek, Rob
• Liskamp, Rob M.J
• Pieters, Roland J

Titel

Multivalent Carbohydrate Recognition on a Glycodendrimer-Functionalized Flow-Through Chip

Ist Teil von

• Chembiochem : a European journal of chemical biology, 2008-07, Vol.9 (11), p.1836-1844

Ort / Verlag

Weinheim: Wiley-VCH Verlag

Erscheinungsjahr

2008

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Dendrimers were fitted out with up to eight mannose moieties by "click" chemistry. They were subsequently attached to aluminum oxide chips via a spacer that was linked to the dendrimer core; this resulted in a microarray of glycodendrimers. Binding of the glycodendrimers to the fluorescent lectins ConA and GNA was observable in real time. In a single experiment it was possible to observe the multivalency enhancement or cluster effect in the binding event. This effect was small for ConA, in agreement with its widely spaced binding sites, whereas it was large for GNA, with its twelve much more closely spaced binding sites. The dendrimer-fitted chip represents a valuable screening tool for multivalency effects. Furthermore kinetic and thermodynamic data on binding events can be deduced. Inhibition experiments are also possible with the system as was shown for ConA with α-methyl mannose as the inhibitor.