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Origin of new genes and source for N-terminal domain of the chimerical gene, jingwei, in Drosophila
Ist Teil von
Gene, 1999-09, Vol.238 (1), p.135-141
Ort / Verlag
Netherlands: Elsevier B.V
Erscheinungsjahr
1999
Quelle
Access via ScienceDirect (Elsevier)
Beschreibungen/Notizen
This paper deals with a general question posed by the origin of new processed chimerical genes: when a new retrosequence inserts into a new genome position, how does it become activated and acquire novel protein function by recruiting new functional domains and regulatory elements?
Jingwei (
jgw), a newly evolved functional gene with a chimerical structure in
Drosophila, provides an opportunity to examine such questions. The source of its exon encoding C-terminal peptide has been identified as an
Adh retrosequence, which extends the concept of exon shuffling from recombination to retroposition as a general molecular mechanism for the origin of a new gene. However, the origin of 5′ exons remains unclear. We examined two hypotheses concerning the origin of these non-
Adh-derived
jgw exons: (i) these exons might originate from a unique genomic sequence that fortuitously evolved a standard intron–exon structure and regulatory sequence for
jgw; (ii) these exons might be a duplicate of an unrelated previously existing gene. Genomic Southern analysis, in conjunction with construction and screening of a genomic bookshelf (sub-library), was conducted in a group of
Drosophila species. The results demonstrated that there are duplicate genes containing the same structure as the recruited portion of
jgw. We name this duplicate gene in
Drosophila teissieri and
Drosophila yakuba and its orthologous gene in
Drosophila melanogaster as
yellow-emperor (
ymp). Thus, the 5′ exons/introns originated from a previously existing gene that provided new modules with specific sub-function to create
jgw.