Details

Autor(en) / Beteiligte

• Sato, Takashi
• Sawaji, Yasunobu
• Matsui, Norimitsu
• Sato, Hiroshi
• Seiki, Motoharu
• Mori, Yo
• Ito, Akira

Titel

Heat Shock Suppresses Membrane Type 1-Matrix Metalloproteinase Production and Progelatinase A Activation in Human Fibrosarcoma HT-1080 Cells and Thereby Inhibits Cellular Invasion

Ist Teil von

• Biochemical and biophysical research communications, 1999-11, Vol.265 (1), p.189-193

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

1999

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Expression of membrane type-1 matrix metalloproteinase (MT1-MMP) is closely correlated with tumor invasiveness. We investigated the effect of hyperthermia on the production of MT1-MMP in human fibrosarcoma HT-1080 cells. Heat shock at 42°C suppressed the production and gene expression of MT1-MMP in HT-1080 cells. Heat shock-induced suppression of MT1-MMP production resulted in the inhibition of progelatinase A (proMMP-2) activation and the increased release of tissue inhibitor of metalloproteinases 2 from cell surface. In addition, in vitro tumor invasion assay in a Matrigel model indicated that heat shock inhibited the invasive activity of HT-1080 cells. These results suggest that heat shock preferentially suppresses the production of MT1-MMP and thereby inhibits proMMP-2 activation, events which subsequently inhibit tumor invasion. Therefore, heat shock shows an anti-invasive effect along with the known mechanism of inhibiting tumor growth.