Details

Autor(en) / Beteiligte

• Martinez, Xavier
• Li, Xiaomao
• Kovarik, Jiri
• Klein, Michel
• Lambert, Paul‐Henri
• Siegrist, Claire‐Anne

Titel

Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice

Ist Teil von

• European journal of immunology, 1999-10, Vol.29 (10), p.3390-3400

Ort / Verlag

Weinheim: WILEY‐VCH Verlag GmbH

Erscheinungsjahr

1999

Quelle

Wiley-Blackwell Full Collection

Beschreibungen/Notizen

• Early life responses to respiratory syncytial virus (RSV)‐F DNA and RSV‐F protein immunization were studied in murine models of neonatal immunization. RSV‐F DNA induced similar antibody (Ab) responses, antigen‐specific IFN‐γ production and cytotoxic T lymphocyte (CTL) responses in 1‐week‐old and adult BALB / c mice. In contrast, RSV‐F protein induced much higher IL‐5 responses in early life. Both vaccines elicited Ab and CTL responses in spite of maternal Ab, but with distinctive kinetics. Sequential RSV‐F DNA priming / protein boosting primed 1‐week‐old mice for RSV‐F‐specific CTL responses, reduced IL‐5 production and enhanced Ab responses. In contrast, IL‐5 exceeded IFN‐γ responses when young mice were primed with protein and boosted with DNA. Last, when protein and DNA immunization were combined, a single vaccine dose induced early Ab responses, preferential IL‐5 responses but strong CTL responses. Sequential or combined DNA / protein immunization thus represent interesting strategies for early life immunization.