Details

Autor(en) / Beteiligte

• Villaverde, Antonio
• Feliu, Jordi X.
• Arís, Anna
• Harbottle, Richard P.
• Benito, Antoni
• Coutelle, Charles

Titel

A cell adhesion peptide from foot-and-mouth disease virus can direct cell targeted delivery of a functional enzyme

Ist Teil von

• Biotechnology and bioengineering, 1998-08, Vol.59 (3), p.294-301

Ort / Verlag

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

Erscheinungsjahr

1998

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• The G‐H loop of foot‐and‐mouth disease virus is a disordered protrusion of the VP1 protein exposed on the virion surface. This short stretch includes an arginine‐glycine‐aspartic acid tripeptide, a recognized integrin‐binding motif, which is responsible for cell attachment and infection. Eight copies of a peptide reproducing the amino acid sequence of this FMDV ligand have been displayed in solvent‐exposed regions on an enzymatically active recombinant β‐galactosidase. This viral peptide segment enables the chimeric enzyme to bind mammalian cell lines with different efficiencies, probably depending on the number of suitable cell receptors present on each of them. Moreover, it also promotes the internalization of the attached enzyme, which is transiently active inside the cells. These results suggest further exploration of the potential use of short adhesion peptides of viral origin as cell attachment tags to direct the targeted delivery of both genes and enzymes, instead of whole, infectious viruses. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:294–301, 1998.