Details

Autor(en) / Beteiligte

• Allen, David L
• Cleary, Allison S
• Speaker, Kristin J
• Lindsay, Sarah F
• Uyenishi, Jill
• Reed, Jason M
• Madden, Molly C
• Mehan, Ryan S

Titel

Myostatin, activin receptor IIb, and follistatin-like-3 gene expression are altered in adipose tissue and skeletal muscle of obese mice

Ist Teil von

• American journal of physiology: endocrinology and metabolism, 2008-05, Vol.294 (5), p.E918-E927

Ort / Verlag

United States: American Physiological Society

Erscheinungsjahr

2008

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Department of Integrative Physiology, University of Colorado at Boulder, Boulder, Colorado Submitted 31 December 2007 ; accepted in final form 9 March 2008 Myostatin (MSTN) is a secreted growth inhibitor expressed in muscle and adipose. We sought to determine whether expression of MSTN, its receptor activin RIIb (ActRIIb), or its binding protein follistatin-like-3 (FSTL3) are altered in subcutaneous or visceral adipose or in skeletal muscle in response to obesity. MSTN and ActRIIb mRNA levels were low in subcutaneous (SQF) and visceral fat (VF) from wild-type mice but were 50- to 100-fold higher in both SQF and VF from ob/ob compared with wild-type mice. FSTL3 mRNA levels were increased in SQF but decreased in VF in ob/ob compared with wild-type mice. Moreover, MSTN mRNA levels were twofold greater in tibialis anterior (TA) from ob/ob mice, whereas ActRIIb and FSTL3 mRNA levels were unchanged. MSTN mRNA levels were also increased in TA and SQF from mice on a high-fat diet. Injection of ob/ob mice with recombinant leptin caused FSTL3 mRNA levels to decrease in both VF and SQF in ob/ob mice; MSTN and ActRIIb mRNA levels tended to decrease only in VF. Finally, MSTN mRNA levels and promoter activity were low in adipogenic 3T3-L1 cells, but an MSTN promoter-reporter construct was activated in 3T3-L1 cells by cotransfection with the adipogenic transcription factors SREBP-1c, C/EBP , and PPAR . These results demonstrate that expression of MSTN and its associated binding proteins can be modulated in adipose tissue and skeletal muscle by chronic obesity and suggest that alterations in their expression may contribute to the changes in growth and metabolism of lean and fat tissues occurring during obesity. leptin; 3T3-L1 cells; visceral adipose tissue; subcutaneous adipose tissue Address for reprint requests and other correspondence: D. L. Allen, Dept. of Integrative Physiology, Univ. of Colorado at Boulder, Campus Box 354, Boulder, CO 80309 (e-mail: allendl{at}colorado.edu )