Details

Autor(en) / Beteiligte

• Terreno, Enzo
• Sanino, Alberto
• Carrera, Carla
• Castelli, Daniela Delli
• Giovenzana, Giovanni B.
• Lombardi, Alberto
• Mazzon, Roberta
• Milone, Luciano
• Visigalli, Massimo
• Aime, Silvio

Titel

Determination of water permeability of paramagnetic liposomes of interest in MRI field

Ist Teil von

• Journal of inorganic biochemistry, 2008-05, Vol.102 (5-6), p.1112-1119

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2008

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• The water permeability of various liposome membranes has been determined at 298K by measuring the NMR longitudinal water proton relaxation rate of vesicles encapsulating the clinically approved Gd-HPDO3A complex (HPDO3A=10-(2-hydroxypropyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid). Two basic formulations based on DPPC (dipalmitoylphosphatidylcholine) and POPC (palmitoyl-oleylphosphatidylcholine) phospholipids were selected and investigated. Furthermore, the permeability changes caused by the membrane incorporation of amphiphiles like cholesterol and/or metal complexes of interest for designing improved liposome-based MRI contrast agents, were also investigated. The incorporation of cholesterol and metal complexes bearing C18 saturated chains in POPC-based liposomes reduces the water diffusivity across the membrane bilayer. On the contrary, the incorporation of a macrocyclic metal complex bearing four C12 alkylic chains, one for each coordination arm of the ligand, considerably enhances the water permeability in DPPC-based liposomes. Finally, it is reported that the permeability of POPC-based bilayer is increased when the liposomes are subjected to an osmotic stress.