Journal of cell science, 2006-11, Vol.119 (21), p.4499-4509
2006
Details
- Autor(en) / Beteiligte
- Titel
- Versican-thrombospondin-1 binding in vitro and colocalization in microfibrils induced by inflammation on vascular smooth muscle cells
- Ist Teil von
- Journal of cell science, 2006-11, Vol.119 (21), p.4499-4509
- Ort / Verlag
- England: The Company of Biologists Limited
- Erscheinungsjahr
- 2006
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We identified a specific interaction between two secreted proteins, thrombospondin-1 and versican, that is induced during a toll-like receptor-3-dependent inflammatory response in vascular smooth muscle cells. Thrombospondin-1 binding to versican is modulated by divalent cations. This interaction is mediated by interaction of the G1 domain of versican with the N-module of thrombospondin-1 but only weakly with the corresponding N-terminal region of thrombospondin-2. The G1 domain of versican contains two Link modules, which are known to mediate TNFα-stimulated gene-6 protein binding to thrombospondin-1, and the related G1 domain of aggrecan is also recognized by thrombospondin-1. Therefore, thrombospondin-1 interacts with three members of the Link-containing hyaladherin family. On the surface of poly-I:C-stimulated vascular smooth muscle cells, versican organizes into fibrillar structures that contain elastin but are largely distinct from those formed by hyaluronan. Endogenous and exogenously added thrombospondin-1 incorporates into these structures. Binding of exogenous thrombospondin-1 to these structures, to purified versican and to its G1 domain is potently inhibited by heparin. At higher concentrations, exogenous thrombospondin-1 delays the poly-I:C induced formation of structures containing versican and elastin, suggesting that thrombospondin-1 negatively modulates this component of a vascular smooth muscle inflammatory response.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9533eISSN: 1477-9137DOI: 10.1242/jcs.03171Titel-ID: cdi_proquest_miscellaneous_69021054
- Format
- –
- Schlagworte
- Aggrecans - metabolism, Animals, Aorta - cytology, Aorta - metabolism, Blood Platelets - metabolism, Elastin - metabolism, Fluorescent Antibody Technique, Humans, Immunoassay, In Vitro Techniques, Inflammation, Mice, Microfibrils - metabolism, Muscle, Smooth, Vascular - cytology, Myocytes, Smooth Muscle - metabolism, Poly I-C - metabolism, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - genetics, RNA, Messenger - metabolism, Thrombospondin 1 - genetics, Thrombospondin 1 - metabolism, Thrombospondins - metabolism, Toll-Like Receptor 3 - metabolism, Versicans - genetics, Versicans - metabolism