Details

Autor(en) / Beteiligte

• Fantozzi, Anna
• Ermolli, Monica
• Marini, Massimiliano
• Scotti, Domenico
• Balla, Branko
• Querci, Maddalena
• Langrell, Stephen R. H
• Van den Eede, Guy

Titel

First Application of a Microsphere-Based Immunoassay to the Detection of Genetically Modified Organisms (GMOs):  Quantification of Cry1Ab Protein in Genetically Modified Maize

Ist Teil von

• Journal of agricultural and food chemistry, 2007-02, Vol.55 (4), p.1071-1076

Ort / Verlag

Washington, DC: American Chemical Society

Erscheinungsjahr

2007

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• An innovative covalent microsphere immunoassay, based on the usage of fluorescent beads coupled to a specific antibody, was developed for the quantification of the endotoxin Cry1Ab present in MON810 and Bt11 genetically modified (GM) maize lines. In particular, a specific protocol was developed to assess the presence of Cry1Ab in a very broad range of GM maize concentrations, from 0.1 to 100% [weight of genetically modified organism (GMO)/weight[. Test linearity was achieved in the range of values from 0.1 to 3%, whereas fluorescence signal increased following a nonlinear model, reaching a plateau at 25%. The limits of detection and quantification were equal to 0.018 and 0.054%, respectively. The present study describes the first application of quantitative high-throughput immunoassays in GMO analysis. Keywords: GMO; immunoassay; Cry1Ab; high-throughput analysis; quantitative detection assay; fluorescent beads