Details

Autor(en) / Beteiligte

• Kim, S.J. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea)
• Lee, C.M. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea)
• Kim, M.Y. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea)
• Yeo, Y.S. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea)
• Yoon, S.H. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea)
• Kang, H.C. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea)
• Koo, B.S. (National Institute of Agricultural Biotechnology, RDA, Suwon, Republic of Korea), E-mail: bskoo@rda.go.kr

Titel

Screening and Characterization of an Enzyme with β-Glucosidase Activity from Environmental DNA

Ist Teil von

• Journal of microbiology and biotechnology, 2007-06, Vol.17 (6), p.905-912

Ort / Verlag

Seoul: Korean Society for Applied Microbiology

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• A novel β-glucosidase gene, bglA, was isolated from uncultured soil bacteria and characterized. Using genomic libraries constructed from soil DNA, a gene encoding a protein that hydrolyzes a fluorogenic analog of cellulose, 4-methylumbelliferyl β-D-cellobioside (MUC), was isolated using a microtiter plate assay. The gene, bglA, was sequenced using a shotgun approach, and expressed in E. coli. The deduced 55-kDa amino acid sequence for bglA showed a 56% identity with the family 1 glycosyl hydrolase Chloroflexus aurantiacus.