Details

Autor(en) / Beteiligte

• Cheng, Lei
• Qiu, Tian-Lei
• Yin, Xiao-Bo
• Wu, Xiao-Lei
• Hu, Guo-Quan
• Deng, Yu
• Zhang, Hui

Titel

Methermicoccus shengliensis gen. nov., sp. nov., a thermophilic, methylotrophic methanogen isolated from oil-production water, and proposal of Methermicoccaceae fam. nov

Ist Teil von

• International journal of systematic and evolutionary microbiology, 2007-12, Vol.57 (12), p.2964-2969

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• 1 Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China 2 Center for Environmental Biotechnology, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China Correspondence Hui Zhang zhanghuits{at}yahoo.com.cn A thermophilic, methylotrophic methanogen, strain ZC-1 T , was isolated from the Shengli oilfield, China. Cells of strain ZC-1 T were motile cocci, 0.7–1.0 µm in diameter and always occurred in clusters of two to four cells. Lysis-susceptibility experiments and analysis of transmission electron micrographs of strain ZC-1 T suggested the presence of a proteinaceous cell wall. Strain ZC-1 T used methanol, methylamine and trimethylamine as substrates for methanogenesis. Optimal growth, with a doubling time of around 5 h, occurred at pH 6.0–6.5, 65 °C, 0.3–0.5 M NaCl and 0.05–0.20 M MgCl 2 . The DNA G+C content of this organism was 56 mol%. Analysis of 16S rRNA gene sequence and the inferred amino acid sequence of the mcrA gene of strain ZC-1 T indicated that it is related specifically to members of the family Methanosaetaceae (90.6 and 76.6 % sequence similarity, respectively). However, strain ZC-1 T failed to grow with acetate as substrate for methanogenesis, which is a special characteristic of the family Methanosaetaceae . Based on these phenotypic and phylogenic characteristics, strain ZC-1 T is proposed to represent a novel genus and species, for which the name Methermicoccus shengliensis gen. nov., sp. nov. is proposed. The type strain is ZC-1 T (=CGMCC 1.5056 T =DSM 18856 T ). Methermicoccaceae fam. nov. is also proposed. Abbreviations: HS-coM, coenzyme M The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and mcrA gene sequences of strain ZC-1 T are DQ787474 and EF026570 , respectively.