Details

Autor(en) / Beteiligte

• Abdulaev, Najmoutin G
• Ngo, Tony
• Ramon, Eva
• Brabazon, Danielle M
• Marino, John P
• Ridge, Kevin D

Titel

The Receptor-Bound “Empty Pocket” State of the Heterotrimeric G-Protein α-Subunit Is Conformationally Dynamic

Ist Teil von

• Biochemistry (Easton), 2006-10, Vol.45 (43), p.12986-12997

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• Heterotrimeric G-protein activation by a G-protein-coupled receptor (GPCR) requires the propagation of structural signals from the receptor-interacting surfaces to the guanine nucleotide-binding pocket. To probe conformational changes in the G-protein α-subunit (Gα) associated with activated GPCR (R*) interactions and guanine nucleotide exchange, high-resolution solution NMR methods are being applied in studying signaling of the G-protein, transducin, by light-activated rhodopsin. Using these methods, we recently demonstrated that an isotope-labeled Gα reconstituted heterotrimer forms functional complexes under NMR experimental conditions with light-activated, detergent-solubilized rhodopsin and a soluble mimic of R*, both of which trigger guanine nucleotide exchange [Ridge, K. D., et al. (2006) J. Biol. Chem. 281, 7635−7648]. Here, it is shown that both light-activated rhodopsin and the soluble mimic of R* form trapped intermediate complexes with a GDP-released “empty pocket” state of the heterotrimer in the absence of GTP (or GTPγS). In contrast to guanine nucleotide-bound forms of Gα, the NMR spectra of the GDP-released, R*-bound empty pocket state of Gα display severe line broadening suggestive of a dynamic intermediate state. Interestingly, the conformation of a GDP-depleted, Mg2+-bound state of Gα generated in a manner independent of R* does not exhibit a similar degree of line broadening but rather appears structurally similar to the GDP/Mg2+-bound form of the protein. Taken together, these results suggest that R*-mediated changes in the receptor-interacting regions of Gα, and not the absence of bound guanine nucleotide, are the predominant factors which dictate Gα conformation and dynamics in this R*-bound state of the heterotrimer.