Details

Autor(en) / Beteiligte

• Saito, Masako
• Kawana, Junichi
• Ohno, Tetsuro
• Kaneko, Masahiro
• Mihara, Kiyoshi
• Hanada, Kazuhiko
• Sugita, Risa
• Okada, Natsuki
• Oosato, Sachiko
• Nagayama, Masatoshi
• Sumiyoshi, Tetsuya
• Ogata, Hiroyasu

Titel

Enantioselective and highly sensitive determination of carvedilol in human plasma and whole blood after administration of the racemate using normal-phase high-performance liquid chromatography

Ist Teil von

• Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2006-10, Vol.843 (1), p.73-77

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2006

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• A highly sensitive HPLC method for enantioselective determination of carvedilol in human whole blood and plasma was developed. Carvedilol and S-carazolol as an internal standard extracted from whole blood or plasma were separated using an enantioselective separation column (Chiralpak AD column; 2.0 Ø × 250 mm) without any chiral derivatizations. The mobile phase was hexane:isopropanol:diethylamine (78:22:1, v/v). The excitation and emission wavelengths were set at 284 and 343 nm, respectively. The limits of quantification for the S(−)- and R(+)-carvedilol enantiomers in plasma and blood were both 0.5 ng/ml. Intra- and inter-day variations were less than 5.9%. As an application of the assay, concentrations of carvedilol enantiomer in plasma and blood samples from 15 patients treated with carvedilol for congestive heart failure were determined.