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Chromatin supraorganization and extensibility in mouse hepatocytes with development and aging
Cytometry. Part A, 2007-01, Vol.71A (1), p.28-37
Moraes, Alberto S.
Guaraldo, Ana Maria A.
Mello, Maria Luiza S.
2007
Details
Autor(en) / Beteiligte
Moraes, Alberto S.
Guaraldo, Ana Maria A.
Mello, Maria Luiza S.
Titel
Chromatin supraorganization and extensibility in mouse hepatocytes with development and aging
Ist Teil von
Cytometry. Part A, 2007-01, Vol.71A (1), p.28-37
Ort / Verlag
Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Erscheinungsjahr
2007
Link zum Volltext
Quelle
Wiley Online Library Journals Frontfile Complete
Beschreibungen/Notizen
Background: Chromatin supraorganization and extensibility, which lead to the formation of extended chromatin fibers (ECF), are affected by starvation and refeeding in adult mouse hepatocytes. It is expected that they could also change with mouse development and aging. Methods: Methods used involved topochemistry, image analysis, microspectrophotometry, gravity action, and polarization microscopy. Results: Increased nuclear areas and Feulgen‐DNA amounts with advancing hepatocyte polyploidy were found with development and aging. A slightly less packed chromatin with more heterogeneously distributed condensation levels was detected in young and old mice. Con‐A responsiveness was almost absent in young mice but very deep in aged mice. ECFs formed from nuclei of adult and aged mice but not from nuclei of young mice. The frequency of ECF formation with the long lysis protocol increased with aging. Conclusions: In young mice, a less packed chromatin state may be associated with more intense gene activity, thus increasing the DNA‐nuclear matrix interactions, and inhibiting ECF formation. Reduced DNA‐nuclear matrix interactions besides defects in heterochromatin formation may induce higher ECF formation and chromatin unpackaging in old mice. We suggest that differences in Con‐A staining relate to different gene activity with advancing development and aging. © 2007 International Society for Analytical Cytology
Sprache
Englisch
Identifikatoren
ISSN: 1552-4922
eISSN: 1552-4930
DOI: 10.1002/cyto.a.20356
Titel-ID: cdi_proquest_miscellaneous_68957757
Format
–
Schlagworte
aging
,
Aging - physiology
,
Animals
,
Chromatin - physiology
,
chromatin supraorganization
,
Con‐A‐positive glycoproteins
,
development
,
extended chromatin fibers
,
Hepatocytes - cytology
,
Hepatocytes - physiology
,
Male
,
Mice
,
microspectrophotometry
,
polarization microscopy
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