Details

Autor(en) / Beteiligte

• Rodriguez, Annette R
• Arulanandam, Bernard P
• Hodara, Vida L
• McClure, Hazel M
• Cobb, Elaine K
• Salas, Mary T
• White, Robert
• Murthy, Krishna K

Titel

Influence of interleukin-15 on CD8+ natural killer cells in human immunodeficiency virus type 1-infected chimpanzees

Ist Teil von

• Journal of general virology, 2007-02, Vol.88 (2), p.641-651

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• 1 Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78245-0549, USA 2 Department of Biology, University of Texas at San Antonio, San Antonio, TX, USA Correspondence Krishna K. Murthy kmurthy{at}sfbr.org Chimpanzees are susceptible to human immunodeficiency virus type-1 (HIV-1) and develop persistent infection but generally do not progress to full-blown AIDS. Several host and immunological factors have been implicated in mediating resistance to disease progression. Chimpanzees have a higher prevalence of circulating natural killer (NK) cells than humans; however, their role in mediating resistance to disease progression is not well understood. Furthermore, NK cell survival and activity have been shown to be dependent on interleukin-15 (IL-15). Accordingly, the influence of IL-15 on NK cell activity and gamma interferon (IFN- ) production was evaluated in naive and HIV-1-infected chimpanzees. In vitro stimulation of whole-blood cultures with recombinant gp120 (rgp120) resulted in enhanced IFN- production predominantly by the CD3 – CD8 + subset of NK cells, and addition of anti-IL-15 to the system decreased IFN- production. Moreover, in vitro stimulation with recombinant IL-15 (rIL-15) augmented IFN- production from this subset of NK cells and increased NK cell cytotoxic activity. Stimulation with rgp120 also resulted in a 2- to 7-fold increase in IL-15 production. These findings suggest that chimpanzee CD3 – CD8 + NK cells play a vital role in controlling HIV-1 infection by producing high levels of IFN- , and that IL-15 elicits IFN- production in this subpopulation of NK cells in HIV-1-infected chimpanzees.