Bioconjugate chemistry, 2006-09, Vol.17 (5), p.1109-1115
2006
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Details
- Autor(en) / Beteiligte
- Titel
- HER2 Specific Tumor Targeting with Dendrimer Conjugated Anti-HER2 mAb
- Ist Teil von
- Bioconjugate chemistry, 2006-09, Vol.17 (5), p.1109-1115
- Ort / Verlag
- United States: American Chemical Society
- Erscheinungsjahr
- 2006
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- In the present study, we report the synthesis and human growth factor receptor-2 (HER2) specific tumor targeting properties of a dendrimer conjugated to anti-HER2 mAb (monoclonal antibody) conjugate. The polyamidoamine (PAMAM) dendrimer generation five (G5) was labeled with alexaFluor 488 and conjugated to anti-HER2 mAb. The binding and internalization of the antibody-conjugated dendrimer to HER2-expressing cells was evaluated by flow cytometry and confocal microscopy. Uniquely, the conjugate demonstrated cellular uptake and internalization in HER2-expressing cells as compared to free antibody. The time course of internalization and blocking experiments with free antibody suggest that the rapid and efficient cellular internalization of the dendrimer−antibody conjugate was achieved without alterations in specificity of targeting. Animal studies demonstrated that the conjugate targets HER2-expressing tumors.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1043-1802eISSN: 1520-4812DOI: 10.1021/bc050348pTitel-ID: cdi_proquest_miscellaneous_68870988
- Format
- –
- Schlagworte
- Animals, Antibodies, Monoclonal - chemistry, Antibodies, Monoclonal - metabolism, Biocompatible Materials - chemistry, Biocompatible Materials - metabolism, Cell Line, Cellular biology, Chemical synthesis, Dendrimers, Female, Fluorescent Dyes - chemistry, Fluorescent Dyes - metabolism, Human growth, Humans, Materials Testing, Mice, Mice, SCID, Microscopy, Molecular Structure, Molecules, Neoplasms - metabolism, Polyamines - chemistry, Polyamines - metabolism, Receptor, ErbB-2 - chemistry, Receptor, ErbB-2 - immunology, Receptor, ErbB-2 - metabolism, Transplantation, Heterologous, Tumors