Science (American Association for the Advancement of Science), 2006-09, Vol.313 (5793), p.1632-1636
2006
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- Autor(en) / Beteiligte
- Titel
- C-Terminal Signal Sequence Promotes Virulence Factor Secretion in Mycobacterium tuberculosis
- Ist Teil von
- Science (American Association for the Advancement of Science), 2006-09, Vol.313 (5793), p.1632-1636
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2006
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- Mycobacterium tuberculosis uses the ESX-1/Snm system [early secreted antigen 6 kilodaltons (ESAT-6) system 1/secretion in mycobacteria] to deliver virulence factors into host macrophages during infection. Despite its essential role in virulence, the mechanism of ESX-1 secretion is unclear. We found that the unstructured C terminus of the CFP-10 substrate was recognized by Rv3871, a cytosolic component of the ESX-1 system that itself interacts with the membrane protein Rv3870. Point mutations in the signal that abolished binding of CFP-10 to Rv3871 prevented secretion of the CFP-10 (culture filtrate protein, 10 kilodaltons)/ESAT-6 virulence factor complex. Attachment of the signal to yeast ubiquitin was sufficient for secretion from M. tuberculosis cells, demonstrating that this ESX-1 signal is portable.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1131167Titel-ID: cdi_proquest_miscellaneous_68862157
- Format
- –
- Schlagworte
- Adenosine triphosphatases, Amino Acid Sequence, Amino acids, Antigens, Bacterial - chemistry, Antigens, Bacterial - metabolism, Bacteria, Bacterial Proteins - chemistry, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Bacteriology, Biological and medical sciences, Dimerization, Fundamental and applied biological sciences. Psychology, Membrane Proteins - metabolism, Microbiology, Models, Biological, Molecular Sequence Data, Mutation, Mycobacterium tuberculosis, Mycobacterium tuberculosis - genetics, Mycobacterium tuberculosis - metabolism, Mycobacterium tuberculosis - pathogenicity, Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains, Plasmids, Protein Binding, Protein Sorting Signals, Protein Structure, Tertiary, Protein Transport, Proteins, Recombinant Fusion Proteins - metabolism, Secretion, Signal transduction, Tuberculosis, Two-Hybrid System Techniques, Ubiquitin - metabolism, Ubiquitins, Virulence factors, Virulence Factors - chemistry, Virulence Factors - metabolism, Yeast, Yeasts