Details

Autor(en) / Beteiligte

• Omori, K.
• Valiente, L.
• Orr, C.
• Rawson, J.
• Ferreri, K.
• Todorov, I.
• Medicherla, S.
• Protter, A.A.
• Schreiner, G.F.
• Riggs, A.D.
• Kandeel, F.
• Mullen, Y.

Titel

Inhibition of p38 Mitogen-Activated Protein Kinase Protects Human Islets From Cryoinjury and Improves the Yield, Viability, and Quality of Frozen-Thawed Islets

Ist Teil von

• Transplantation proceedings, 2005-10, Vol.37 (8), p.3422-3423

Ort / Verlag

New York, NY: Elsevier Inc

Erscheinungsjahr

2005

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The development of an optimal islet cryopreservation method will permit transplantation of islets from multiple donors in a single procedure and contribute to alleviation of the islet shortage. In this study, we have improved human islet cryopreservation methods under serum-free conditions using an intracellular-based islet cryopreservation solution (ICS), especially supplemented with a p38 pathway inhibitor (p38IH) to suppress p38 mitogen-activated protein kinase (MAPK) activation. Three different solutions were compared for freezing and thawing of human islets (1) conventional RPMI1640 medium, (2) ICS, and (3) ICS supplemented with a p38IH, SD-282 (ICS-p38IH). Islet cryopreservation with ICS-p38IH significantly improved islet recovery, viability, and quality after thawing of cryopreserved islets. This improvement may allow the use of cryopreserved islets in clinical islet transplantation.