Details

Autor(en) / Beteiligte

• SUGANUMA, Ryota
• PELCZAR, Pawel
• SPETZ, Jean Francois
• HOHN, Barbara
• YANAGIMACHI, Ryuzo
• MOISYADI, Stefan

Titel

Tn5 Transposase-Mediated Mouse Transgenesis

Ist Teil von

• Biology of reproduction, 2005-12, Vol.73 (6), p.1157-1163

Ort / Verlag

Madison, WI: Society for the Study of Reproduction

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• We have developed a novel method for mouse transgenesis. The procedure relies on a hyperactive Tn5 transposase to insert a transgene into mouse chromosomes during intracytoplasmic sperm injection. This procedure integrates foreign DNA into the mouse genome with dramatically increased effectiveness as compared to conventional methods such as pronuclear microinjection and traditional sperm injection-mediated transgenesis. Our data indicate that with this method, transgenic mice, both hybrids and inbreds, can be produced more consistently and with lower numbers of manipulated oocytes required for traditional microinjection methods. The transposase-mediated transgenesis technique is also effective with round spermatids, offering the potential for rescuing the fertility of azoospermic animals using sperm precursor cells. Abstract A novel method of active ICSI transgenesis using a transposase enzyme as the mediator of transgene insertion enables more effective transgenesis with fewer oocytes used