Details

Autor(en) / Beteiligte

• Lancman, Joseph J.
• Caruccio, Nicholas C.
• Harfe, Brian D.
• Pasquinelli, Amy E.
• Schageman, Jeoffrey J.
• Pertsemlidis, Alexander
• Fallon, John F.

Titel

Analysis of the regulation of lin‐41 during chick and mouse limb development

Ist Teil von

• Developmental dynamics, 2005-12, Vol.234 (4), p.948-960

Ort / Verlag

New York: Wiley‐Liss, Inc

Erscheinungsjahr

2005

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• We have cloned the chicken and mouse orthologues of the Caenorhabditis elegans heterochronic gene lin‐41. During limb development, lin‐41 is expressed in three phases over developmental time and most notably is associated with the developing autopod. Using chicken and mouse mutants and bead implantations, we report that lin‐41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways. In C. elegans, it is proposed that lin‐41 activity is temporally regulated by miRNAs (let‐7 and lin‐4) that bind to complementary sites in the lin‐41 3′‐untranslated region (UTR). Taking a bioinformatics approach, we also report the presence of potential miRNA binding sites in the 3′‐UTR of chicken lin‐41, including sites for the chicken orthologues of both C. elegans let‐7 and lin‐4. Finally, we show that these miRNAs and others are expressed in the chick limb consistent with the hypothesis that they regulate chicken Lin‐41 activity in vivo. Developmental Dynamics 234:948–960, 2005. © 2005 Wiley‐Liss, Inc.