Analytical biochemistry, 2006-09, Vol.356 (1), p.94-99
2006
Details
- Autor(en) / Beteiligte
- Titel
- A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays
- Ist Teil von
- Analytical biochemistry, 2006-09, Vol.356 (1), p.94-99
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2006
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatibility with standard methods for protein assays. This feature greatly expands the range of applications for assay systems quantifying the expression of multiple luciferase reporters.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0003-2697eISSN: 1096-0309DOI: 10.1016/j.ab.2006.04.046Titel-ID: cdi_proquest_miscellaneous_68764197
- Format
- –
- Schlagworte
- Animals, Dual-Luciferase, Enzyme Inhibitors - pharmacology, Fireflies - enzymology, Fireflies - genetics, Firefly, Genes, Reporter, HeLa Cells, Humans, Luciferases, Firefly - analysis, Luciferases, Firefly - antagonists & inhibitors, Luciferases, Firefly - genetics, Luciferases, Renilla - analysis, Luciferases, Renilla - genetics, Luminescent Measurements - methods, Photinus, Photinus luciferase, Protein assay, Renilla, Renilla - enzymology, Renilla - genetics, Renilla luciferase