Details

Autor(en) / Beteiligte

• Oluwayelu, D. O
• Todd, D
• Ball, N. W
• Scott, A. N J
• Oladele, O. A
• Emikpe, B. O
• Fagbohun, O. A
• Owoade, A. A
• Olaleye, O. D

Titel

Isolation and Preliminary Characterization of Chicken Anemia Virus from Chickens in Nigeria

Ist Teil von

• Avian diseases, 2005-09, Vol.49 (3), p.446-450

Ort / Verlag

United States: American Association of Avian Pathologists, Inc

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• Chicken anemia virus (CAV) was isolated for the first time from the Nigerian chicken population. The virus was recovered from necropsied birds from broiler and pullet flocks that suffered disease outbreaks tentatively diagnosed as infectious bursal disease. A sensitive polymerase chain reaction (PCR) assay detected CAV DNA in tissues of necropsied birds. Restriction endonuclease analysis performed with the 733-bp PCR product and the Cfo I enzyme indicated at least two different CAVs were circulating among the Nigerian chicken population. Four isolates were obtained from pooled liver and thymus tissues using the MDCC–MSB1 cell line. These isolates were found to be antigenically closely related to the Cuxhaven-1 (Cux-1) reference strain of CAV when reacted with four monoclonal antibodies prepared against the Cux-1 virus. One of the isolates (isolate A) induced thymus atrophy, bone marrow aplasia, and low hematocrit values when inoculated into 1-day-old specific-pathogen-free chickens. These findings not only demonstrate that CAV is present in Nigeria, but they also likely represent the first cell culture isolation of the virus in Africa.