Journal of the American Chemical Society, 2005-11, Vol.127 (43), p.14976-14977
2005
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Details
- Autor(en) / Beteiligte
- Titel
- Structural Characterization of a p-Acetylphenylalanyl Aminoacyl-tRNA Synthetase
- Ist Teil von
- Journal of the American Chemical Society, 2005-11, Vol.127 (43), p.14976-14977
- Ort / Verlag
- Washington, DC: American Chemical Society
- Erscheinungsjahr
- 2005
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- It has been recently shown that orthogonal tRNA/aminoacyl-tRNA synthetase pairs can be evolved to allow genetic incorporation of unnatural amino acids into proteins in both prokaryotes and eukaryotes. Here we describe the crystal structure of an evolved aminoacyl-tRNA synthetase that charges the unnatural amino acid p-acetylphenylalanine. Molecular recognition is due to altered hydrogen bonding and packing interactions with bound substrate that result from changes in both side-chain and backbone conformation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0002-7863eISSN: 1520-5126DOI: 10.1021/ja0549042Titel-ID: cdi_proquest_miscellaneous_68731854
- Format
- –
- Schlagworte
- Amino Acyl-tRNA Synthetases - chemistry, Amino Acyl-tRNA Synthetases - metabolism, Binding Sites, Biological and medical sciences, Crystalline structure, Escherichia coli - enzymology, Fundamental and applied biological sciences. Psychology, Molecular biophysics, Molecular Structure, Phenylalanine - analogs & derivatives, Phenylalanine - chemistry, Protein Conformation, Structure in molecular biology, Tyrosine-tRNA Ligase - chemistry, Tyrosine-tRNA Ligase - metabolism