Cell, 2005-10, Vol.123 (2), p.335-346
2005
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Eater, a Transmembrane Protein Mediating Phagocytosis of Bacterial Pathogens in Drosophila
- Ist Teil von
- Cell, 2005-10, Vol.123 (2), p.335-346
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2005
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Phagocytosis is a complex, evolutionarily conserved process that plays a central role in host defense against infection. We have identified a predicted transmembrane protein, Eater, which is involved in phagocytosis in Drosophila. Transcriptional silencing of the eater gene in a macrophage cell line led to a significant reduction in the binding and internalization of bacteria. Moreover, the N terminus of the Eater protein mediated direct microbial binding which could be inhibited with scavenger receptor ligands, acetylated, and oxidized low-density lipoprotein. In vivo, eater expression was restricted to blood cells. Flies lacking the eater gene displayed normal responses in NF-κB-like Toll and IMD signaling pathways but showed impaired phagocytosis and decreased survival after bacterial infection. Our results suggest that Eater is a major phagocytic receptor for a broad range of bacterial pathogens in Drosophila and provide a powerful model to address the role of phagocytosis in vivo.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0092-8674eISSN: 1097-4172DOI: 10.1016/j.cell.2005.08.034Titel-ID: cdi_proquest_miscellaneous_68714820
- Format
- –
- Schlagworte
- Amino Acid Motifs, Amino Acid Sequence, Animals, Bacteria, Bacterial Infections - prevention & control, Drosophila, Drosophila - cytology, Drosophila - embryology, Drosophila - genetics, Drosophila - microbiology, Drosophila Proteins - genetics, Drosophila Proteins - metabolism, Drosophila Proteins - physiology, Embryo, Nonmammalian, Escherichia coli - pathogenicity, Flow Cytometry, Frameshift Mutation, Genes, Insect, Histidine - chemistry, In Situ Hybridization, Insect Proteins - chemistry, Insect Proteins - genetics, Insect Proteins - metabolism, Insect Proteins - physiology, Macrophages - metabolism, Membrane Proteins - chemistry, Membrane Proteins - genetics, Membrane Proteins - metabolism, Membrane Proteins - physiology, Molecular Sequence Data, Open Reading Frames, Phagocytosis, Receptors, Cell Surface - genetics, Receptors, Cell Surface - metabolism, Receptors, Cell Surface - physiology, RNA Interference, RNA, Messenger - metabolism, Sequence Homology, Amino Acid, Serratia marcescens - pathogenicity